In a study conducted in October 2014, the inhibitory effect of cimenol ring on intestinal pathogen Salmonella invasion was tested.

Procedure

The IPEC-J2 cell line from piglet intestinal tissue was used.

In 9-well plates, 1.2 × 10⁵ cells/ml were added. Once the confluence was observed, the medium was removed and replaced with 2 ml of DMEM supplemented with 0, 1.75 and 5.25 ppm of cimenol.

For each concentration of cimenol, three incubation times were tested: 30, 60 and 90 minutes

After the treatment, a bacterial suspension of Salmonella enteritidis 3934 in DMEM medium was added to each well.

After 45 minutes of infection at 37ºC, the extracellular bacteria were removed, cells were lysed and plated on LB agar to proceed with intracellular bacteria countings.

Results

In all trials showed that intracellular bacteria tend to be reduced when cells are treated with a concentration of 5.25 ppm of cimenol.

The statistical analysis of the data shows that this difference is significant when cells were maintained 90 minutes in the presence of the supplement.

Conclusions

It can be concluded that the cimenol ring, at a concentration of 5.25 ppm, has an effect of inhibition of invasion by Salmonella in porcine intestinal epithelial cells.

The cimenol ring is marketed under the commercial name Alquermold Natural, by Biovet S.A.