Effect of intestinal conditioner pronutrients on intestinal paracellular permeability
Paracellular permeability at the intestinal level depends, as in other epitheliums, on specific junctions between cells known as tight junctions.
Paracellular permeability at the intestinal level depends, as in other epitheliums, on specific junctions between cells known as tight junctions. Tight junctions form a continuous barrier between epithelial cells, needed to separate tissue spaces and regulate the selective movement of molecules through epithelia.
The disturbance of these cell junctions that regulate the paracellular permeability induces a disturbance of the mucosa immune system and inflammation, which can result in clinical symptomatology such as diarrhea or edema.
Intestinal conditioner pronutrients are active molecules of botanical origin capable of enhancing the normal physiological functioning of the intestine and improve the structural integrity of the gut.
Evaluate the effect of intestinal conditioner pronutrients on paracellular permeability at the intestinal level.
Material and methods
The test was performed in vitro. Piglet jejunal tissue cell (IPEC-J2) were used, which were grown in Transwell inserts, with a collagen-coated membrane, a pore size of 0.4 microns and a membrane diameter of 0.65 mm. The cells were grown for 21 days at a density of 5×105 cells/ml, until they reached confluence. The culture medium was renewed every 3 days.
- Control group: cell monolayer without intestinal conditioner pronutrients
- Experimental group: cell monolayer with intestinal conditioner pronutrients at doses of 1:10000 for 90 minutes.
After 90 minutes of treatment in the experimental group, a fluorescein isotiocyanate (FITC) was added at doses of 1 mg/ml at 30, 60, 90 and 120 minutes and the transference of FITC from the apical medium to the basolateral medium was assessed by fluorometry (Biotech Sinergy HT).
The intensity of fluorescence increased in both experimental groups during the samplings, however, in all samples the recorded fluorescence intensity was lower in the case of the intestinal conditioner pronutrient group.
This indicates that the number of fluorescein molecules that crossed the cell monolayer was lower in the case of pronutrient-treated cells, a result that we can relate to a greater integrity of tight cell junctions.
Tight junctions play a key role in paracellular permeability. They allow to maintain the structural integrity of the tissues by regulating the passage of macromolecules.
Alteration of these cell junctions causes physiological alterations related to a disbalance in the transport of molecules, such as edema and diarrhoea, as well as a greater predisposition to disease.
Intestinal conditioning pronutrients allow to strengthen the tight cell junctions contributing to the maintenance of the structural integrity of the gut and thereby improve the protection of animals against exposure to different pathogens.
Intestinal conditioning pronutrients are marketed under the name Alquernat Nebsui from Biovet S.A.