The loss of regenerative capacity of tissues. Disclosure 37
In previous blog we have described the way some pronutrients are able to activate certain genes to give rise to a new organ and in the actual version we will describe how some substances inhibit primitive genetic expressions and in consequence more evolved animals lose primitive capabilities.
Thus, amphibia and reptile have high regenerative capabilities, of their tissues, opposite to mammals.
Recent researches link this phenomenon to the inhibitory action of antioxidants over genes which produce urate oxidase and L-glico-and-lactone oxidase. Both genes are active in mammals embryos in the first phases of gestation but inactive when fetus is formed.
It has been proved that administration of antioxidants to frog tadpoles inhibits transcription factor NF-kB, AP1 and Nrf2, the genes and with them, tail regeneration is inhibited. To evaluate the action of reactive substances to oxygen during tail regeneration, we have to measure H2O2 levels (hydrogen peroxide, a common specie reactive to oxygen in cells), using 2',7'-dichlorofluoresceine diacetate that changes
light emission properties in presence of H2O2.
It is proved that the decrease in levels of reactive to oxygen substances, growth of tissues and regeneration is not produced. In consequence, reactive to oxygen substances are essential to begin and maintain the response to regeneration.